Here a typical example concerning a recent observation of faint star AZ Cas (20 x 600 s sequence on a C9 and
a Lhires III 2400 l/mm, 19-microns slit).
Most important: I have taken one neon lamp spectrum just before starting of the sequence and
one neon lamp spectrum just at the end of the sequence for control (non intermediate neon lamp for the occasion).
Only the individual first neon spectrum is used for spectral calibration under ISIS. ISIS use this unique
spectrum for compute the FWHM (mean FWHM of three available lines). Also, the software use this unique starting
neon lamp spectrum for compute spectral dispersion equation.
Here a typical comparison on the fisrt AZ Cas spectrum and the latest of the sequence (remeber,
all calibrated by using the initial neon spectrum):

The spectral shift (or drift) is evident (here 2.34 pixel of 9.08 size).
Now I activate the spectral shift registration of ISIS (General tab):

The result is better. Also the absolute spectral error is probably minimized
because the reference spectrum (the first of the sequence) is taken
nearly the same time of neon lamp spectrum.
I recommand the procedure for long duration sequence on moderate brigthness
object with sufficient constrasted and numerous stellar spectral line (for an efficient
registration) i.e. F,G,K, ... type stars. Ideal for AZ Cas campaign.
More about ISIS processing of Lhires spectra:
http://www.astrosurf.com/buil/isis/guid ... to1_en.htm
Christian B