Hello,
As planned, did a successful overnight serie of 51 x 5 minute - for now with the 150/mm grating, unfortunately somehow focus was not the best (got finally R~500).
In blue, below 4500 towards 4200A it gets totally defocused (however, above 4600A it's yet healthy).
Here is the result:

- tcrb_20160403_heII4686_var.jpg (53.13 KiB) Viewed 10264 times
In order to understand the final statistics of relative flux measures I made (around 4921A - wanted to use as an extra SNR reference, however continuum is hard to remove):

- tcrb_20160403_4921A_var.jpg (48.87 KiB) Viewed 10264 times
(zeroed in frame@47 the region of 5018A - that was hit by cosmic ray)
Now the final statistics, using IRAF/splot in a semi-scripted manner (= no manual intrvetion, fully reproducible!), of 51 x 5 minute samples:

- tcrb_20160403_51x5min_gnuplot.jpg (123.43 KiB) Viewed 10264 times
For all lines (except the blended set of lines around 4640A) was using splot + 'd' deblend + 'g' gauss fit - continuum, on spectra that was previously brought to the common average 3-order overall continuum.
As for the blend, had to use a dummy flux integration: did splot + 'e' interval above overall 0.85 continuum (gauss fit did not give meaningful data).
To me the HeII 4686 looks changing only.
Other smaller features lost in the complex continuum and low resolution.
Balmer and HeI features are almost restful.
Observation started above 60 degree, let over meridian finishing at approx. 70 degree.
Anyone made similar effort at low res (= sleeping over a night), I'd likely process.
Enjoy!
- Peter
EDIT: re-uploaded smaller jpegs